Summary | Background | Methods | Results | Publications

Results and evaluation

 

Microtubule results and evaluation

Segmented microtubules

(left) selected slices of two volumes respectively. (middle) manual segmentations. (right) automated segmentations


Evaluation by 2D contour area

 

(left) contour overlap ratio indicates the overall agreement between automatic segmentation and manual segmentation. (right) manual and automatic results have the same narrowing and widening trends

 

Evaluation by position of central axes

Axes by manual segmentation and automated segmentation are close to each other. Automated segmentation varies smooth, while manual segmentation varies roughly

 

 

Plus-ends results and evaluation

 

 

Plus-ends traced automatically

Corresponding radial slices



Evaluation by classification

Classification of plus-ends structures is the goal of the biological research behind this work. By comparing classification based on automatic results with that based on raw images, we can estimate how much useful information is obtained through automated tracing. 80 test samples are used: 18 curled, 22 forked and 40 blunt (no filament traced or very short filament) plus-ends.

 

 

Confusion table for three classes of plus-ends

 

True Class ß

Curled

Forked

Blunt

Curled

0.78

0.22

0.00

Forked

0.14

0.68

0.18

Blunt

0.05

0.15

0.80

 

 

Summary | Background | Methods | Results | Publications